Description
Principle of the assay: This assay employs the quantitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CCP antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for CCP present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human anti-CCP antibody bound in the initial step. The color development is stopped and the intensity of the color is measured